AFLP TEKNII PDF

Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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SAS language guide for personal computers. Indeed, we found that the RFLP and AFLP markers produced sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high correlations between these two marker systems, the similarity between the results being explainable by the fact that they are similar techniques teknii on restriction site changes.

Barbosa I ; Isaias O.

The objectives of the study described etknii this paper was: Because the box-plots for each of our groups of samples were skewed we used the mean, median and maximum CV values to determine the adequate number of polymorphic loci needed for acceptable precision.

Rev Bras Genet 4: Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational crosses. Although the AFLP markers gave the lowest mean PIC value they provided a similar degree of polymorphism information content to that provided by the RAPD markers, which agrees with the results published by Becker et al.

In the work of Lanza et al. Detailed descriptions of these populations are given in Lanza et al.

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The Plant J 3: Bootstrap analysis were used to evaluate the number afp loci for the markers and the coefficients of variation CV revealed a skewed distribution. Theor Applied Genet However, in the case of maize, tropical and temperate populations differ from each other because tropical populations usually originate from composites with higher genetic variability and, most of the time, it is difficult to allocate tropical composites to well-defined heterotic groups by phenotypic evaluation.

Introduction The past limitations associated with pedigree data and morphological, physiological and cytological markers for assessing genetic diversity in cultivated and wild plant species have largely been circumvented by the development of DNA markers such as restriction fragment length polymorphisms RFLPs; Botstein et al. Variability in Chinese sorghums.

Microsatellites SSRs occur frequently in most eukaryote genomes and can be very informative, multiallelic and reproducible Vos et al. Informe de Pesquisa The coefficient of variation CV for all genetic distances across teknli bootstrap samples was estimated for each specific number of loci or bands sampled, a computer program for performing these analyses being set up using the ‘RANNUNI’ function of the SAS system Version 8.

Calculations were made using the following formula: Similar patterns were observed for both genetic distance and heterosis. J Genet Breed Correlations between genetic distances measured with different markers.

Results Levels of polymorphism All of the 18 maize inbred lines studied by us had previously been investigated using the four different marker systems RAPD: Pesq Agropec Bras 28, For the AFLP method 20 primer combinations were used and binary scored 1 or 0 with each band being considered a locus while for the SSR method 68 polymorphic primers were used with the binary data being converted into a genotypic matrix which was used to identify alleles and their respective loci.

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teknui

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

European Patent Application number: We used the median and maximum coefficient of variation values to evaluate the accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data for which there is no assurance that the CVs values are distributed symmetrically.

Heterotic group assignment and hybrid performance determined by RFLP marker. For codominant markers, however, the distribution of values within each sample is skewed towards the higher values and it appears that mean or median CV values should be appropriate.

Comparison of the genetic distances generated by different molecular markers in diversity studies have been reported by several authors Hahn et al.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

Although the discrimination power of RFLPs in diversity studies has heknii well documented Smith et al. The loci used were: I ; Anete P. Total genomic DNA was isolated from a bulk of five-week-old leaf tissue taken from 16 plants of each line, then being isolated and purified by the method of Hoisington et al. Bootstrap analysis As expected, the magnitude of the coefficient of variation CV reknii decreased as the number of polymorphic loci bands evaluated increased.