MicroRNA regulate the cell cycle in mast cells. [Immunol Biological principles of microRNA-mediated regulation: Shared themes amid diversity. Biological principles of microRNA-mediated regulation: shared themes amid diversity. Nat Rev Genet. ;9(11)– The mirtron pathway generates microRNA-class regulatory RNAs in Biological principles of microRNA-mediated regulation: shared themes amid diversity.

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Parameter estimation required two sets of precomputed data. China Biotechnology,31 Although the genomes of many individual species bear more than 45 mirtrons e.

Shifted processing and alternate biogenesis pathways of Drosophila miRNAs. Because increasing depths provided minor returns Fig. Among our novel miRNA annotations, a handful of loci appeared to be cloned from a broad range of Drosophilid species Supplemental Fig.

The study shows that there are miRNAs in protozoa and viruses. However, perhaps unexpected was that even in species relatively close to D. However, in the absence of systematic small RNA sequencing analysis across a genus, the tenets of this assumption have not systematically been challenged by empirical data. Nat Genet, ,37 5: The miRNA machinery in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic nevi.

Eric C. Lai – Google Scholar Citations

Using these comprehensive data, we elaborate numerous features of miRNA annotation and evolution, and show how these differ with respect to miRNA biogenesis types, tissues within an animal, and between different branches reghlation the fruit fly phylogeny. F amif I Impact of terminal uridylation system on evolutionary suppression of mirtrons and behavior of canonical miRNAs.


Nucleic Acids Res, theems, Highlighted are examples of seed divergence between expressed TRC miRNA orthologs between these closely related species, consistent with adaptive evolutionary behavior.

The tables are turned when examining the fraction of conserved loci in the other categories of miRNAs. PLoS Comput Aamid Further details and considerations are provided in the Supplemental Text. Altogether, these findings indicate substantial heterogeneity among evolutionary rates of multiple classes of canonical and noncanonical miRNAs and also highlight differential behavior along individual lineages. Article published online before print.

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date see http: These are all signatures of Drosha- and Dicer-mediated cleavage. These data broadly extend the limited collection of publicly available sRNA data from other fly species, primarily D. Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs.

Biological principles of microRNA-mediated regulation: shared themes amid diversity.

The majority of miRNAs identified are singletons species-specific or doubletons clade-specific. Summary of all known and novel miRNAs recovered within 12 Drosophila genomes. It provides browsing and searching function for miRNAs, targetor sequences. Adaptive evolution of testis-specific, recently-evolved, clustered miRNAs in Drosophila. Cell,2: In any case, it is clear that the wholesale appearance and disappearance of extensive TRC loci in different clades reflects a fundamentally different usage of these miRNAs than for maintenance of conserved seed-driven target networks as with typical canonical miRNAs.

Paralogous miRNAs were assigned to single-species alignment. Similarly, the mirtron class also contains very few conserved loci. We solidify the perspective that miRNAs do not comprise a unitary class, but encompass a diversity of functional loci with distinct evolutionary imperatives.


The first datum needed was a binary encoding of miRNA presence 1 and absence 0 as leaf node labels of the phylogenetic model. A microRNA target prediction and functional annotation database with a wiki interface. However, some conserved miRNAs continue to be found, many of which derive from unusual genomic locations or noncanonical pathways, perhaps explaining why they were overlooked earlier. Although data were sampled more deeply in some species e.

A A phylogenetic probabilistic graphical model for estimating rates of gene birth and death. Please wait a minute At the other end of the spectrum, mirtrons exhibited the highest rate estimates. Selective suppression of the splicing-mediated microRNA pathway by the terminal uridyltransferase tailor. Repertoire and evolution of miRNA genes in four divergent nematode species. All hairpin candidates from the pipelines were vetted manually and bioinformatically, and additional considerations are described in the Supplemental Text.

Paralogs were a by-product of this procedure because they attained lower rank during orthology assignments.

Temporal reciprocity of miRNAs and their targets during the maternal-to-zygotic transition in Drosophila. Specifically, two unique iso-miR sequences are each preferentially abundant in the Sophophora group and Drosophila group species, respectively, and for mir regulatioj, the melanogaster group species produces one iso-miR sequence that is distinct from the dominant iso-miR of other Sophophorans.

Adaptive evolution of newly-emerged microRNA genes in Drosophila.