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Electrofusion is carried out by applying both the AC and DC pulse waveforms.
If you continue we assume that you consent to receive all cookies on this website. For use in ,anual production applications, the Legacy ECM generator, 3. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.
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This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.
The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for mm petri dishes. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make cem2001 an extremely versatile system for any lab.
Cell fusion Nuclear transfer Embryo manipulation Hybridoma production Mammalian cell transfection Plant protoplast fusion Stem cell production.
Not for use in diagnostic, pre-clinical, or clinical procedures. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids.
Electrofusion is achieved by the generation of a proprietary 1 MHz Evm2001 waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results.
Multi-purpose electro cell manipulation generator capable of producing a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion. The LEGACY ECM is a multipurpose electro cell manipulation pulse generator capable of performing both mqnual for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications.
Utilizing the Legacy ECM for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device. The Legacy ECM coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing janual.
After fusion, the AC pulse reapplication maintains compression of the cells following the Emc2001 pulse resulting in a higher number of hybrids. Proprietary blend; sterile filtered and made of the highest quality medical-grade chemicals.
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Supplier BTX show all products. Electroporate adherent cells directly into the dish mnual for cell growth. The Legacy ECM is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion.
Microsecond switch-over time from AC to DC allows for highly efficient fusion.
The 1 MHz AC pulse aligns cells together in real time, saving time seconds vs.