Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Proto;lastos conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. The morphology of fusion-derived regenerants was intermediate between the donor parents.

Improvements in regeneration from protoplasts of potato and studies on chromosome stability. Protoplasts were precipitated by centrifugation at rpm during five minutes. A subscription to J o VE is required to view this article. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.

When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking.

Fusion de protoplastos pdf

Both 6carboxyfluorescein and the kanamycin resistance harboured protollastos the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique. Protoplasts were precipitated by centrifugation at rpm during 10 minutes. A slot-blot hybridization method for screening somatic hybrids. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S.


Final concentration was protoplasts per millilitre.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. For the obtained somatic hybrid plants, the chromosome number varied from Table 2.

Centrifugar el tubo a x g por min a protkplastos ambiente para cosechar protoplastos. Shoot regeneration started twelve weeks after calli were transferred to MS medium. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.

Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.

Cultivo y fusion de protoplastos

The numbers of chromosome of S. Fill out the form below to receive a free trial or learn more about access:. Instpp Protoplast fusion, culture and regeneration. The effect of initial culture media. Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type.

Protoplasts fusion from Fueion. For other languages click here. Your institution must protopllastos to JoVE’s Biology section to access this content.

Cultivo y fusion de protoplastos [1990]

Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.

Non-transgenic applications of plant tissue culture in potato.

RAPD and chromosomal analysis of the hybrids are also presented. None of the regenerants had 72 chromosomes, which results from the protoplasts fusion of a tetraploid with a diploid genome.


The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis.

For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications. In Vitro Cell Dev. These results showed that all hybrids were aneuploids Fig.

These plants were multiplied in glass culture tubes for further analysis. Universidad Nacional Agraria La Molina.

This value is different from that obtained by Mattheij et al. Somatic hybridization in the genus Solanum: Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. If that doesn’t help, please let us know. These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al. Regeneration of isolated protoplasts to plants in Solanum dulcamara.

For these reasons and considering the obtained results, it can be suggested that there is a high probability of fusion-derived regenerants which are aneuploids with an increase or loss of some chromosomes when one or both fusion partners have a high ploidy level. Isolation of Protoplasts from Tissues of day-old Seedlings of Arabidopsis thaliana …. Fill out the form below to receive a free trial or learn more about access: