Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
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m RealTime System from Abbott | SelectScience
The fluorescence counts were converted into VL measure by the analyzer Abbott, a ; Abbott, However, comparison of the differences of VL measurements between the two methods by using the Bland Altman plot showed rough symmetric distribution of the differences and the log differences in Consistently, comparison of the distribution and median values of VL between these two methods also showed similar median VL values but a slight wider distribution by the Roche method than the Abbott method m0200sp seen Figure 2.
Real-time PCR was used for detection. Together, these data suggest that neither method is better than the other for measuring VL. Finally, the NA was released using an elution buffer.
However, a relative significant percentage However, this factor should not be the major contributing factor because a recent surveillance study at the University of Maryland Medical Center showed that about The number of samples within this range was All VL values were first transformed into the log10 format.
The plot to abvott left represents the distribution of the VL from the Abbott method assay and the plot to the right represents the distribution of Abbbott from the Roche assay. Bland-Altman analysis of the quantitative results. HIV viral particles were first lysed and an HIV-1 Quantitation Standard QS Armored RNA molecule was added to each sample, which was followed by a series of processes which involves incubation, introduction of m200s0p particles, washing to remove unbound particles and elution of nucleic acid NA at elevated temperatures.
Abbott Molecular is committed to molecular diagnostics. All blood samples were collected in EDTA tubes. The general principle for NA extraction is similar to that of the Roche method, i.
Abbottt of the resulting fluorescence collectively provides an immediate real-time quantification of the PCR process. Using the TaqMan analyzer, the sample extraction volume protocol is 0.
Abbott M2000sp Liquid Handler W/ M2000rt PCR
The results showed as. A total of paired plasma samples were tested in the same day by both methods Table 2.
Comparison of the mean VL with differences of the two methods using the Bland Altman plot showed rough symmetric distribution of the differences, indicating neither method is better than the other for measuring VL. For examples, the Abbott method has been shown to have better detectability of non-B subtypes Geelen et al. This trend has lead to the development and approval of a number of diagnostic assays For reviews of these assays, see Constantine and Zhao, In this study, we have compared VL quantification by the Roche and the Abbott methods side-by-side.
The extracted NA was mixed with the amplification reagents before amplification with the mrt Abbott, a ; Abbott, Can’t read the image?
In this configuration, signal is not created by probe hydrolysis but by separation of the probe from quencher oligonucleotide. Babott by real-time technology used two probes of different lengths: The Bland-Altman analysis was used for the analysis of the concordance of the two methods Bland and Altman, The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al.
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Other assay performance characteristics such as analytical specificity, linearity, reproducibility, and precision were not evaluated. The Abbott m RealTime System provides automation from bar-coded laboratory m200sp through patient result, creating an efficient workflow for your laboratory. Thus some of the discordant samples could potentially be due to the HIV-1 subtype differences. It should be mentioned that the genetic diversity of HIV-1 subtypes could present a significant challenge in the accurate VL monitoring of HIV-infected patients who carry non-B subtypes Damond et al.
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